Description
SPRINGER Microfluidic Techniques 2006 Edition by MINTEER, SHELLEY D.
Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Overview of Advances in Microfluidics and MicrofabricationShelley D. Minteer and Christine M. MoorePart I. Microfabrication MethodsIntroduction to Microfabrication TechniquesRabih Zaouk, Benjamin Y. Park, and Marc J. MadouFabrication of Polydimethylsiloxane Microfluidics Using SU-8 MoldsRabih Zaouk, Benjamin Y. Park, and Marc J. MadouFabrication of Microelectrodes Using the Lift-Off TechniqueBenjamin Y. Park, Rabih Zaouk, and Marc J. MadouLaser Ablation as a Fabrication Technique for Microfluidic DevicesEmanuel A. WaddellReversible, Room Temperature Bonding of Glass Devices for MicrofluidicsLoranelle L. LockyearPart II. Biological ApplicationsSolid-Phase Extraction in Packed Beds on Glass MicrodevicesLoranelle L. LockyearFabrication of Micromachined Magnetic Particle Separators for Bioseparation in Microfluidic SystemsJin-Woo ChoiHydrogel-Immobilized Antibodies for Microfluidic Immunoassays: Hydrogel ImmunoassayGloria Thomas, Emad M. El-Giar, Laurie E. Locascio, and Michael J. TarlovPolymerase Chain Reaction in Miniaturized Systems: Big Progress in Little DevicesKalee D. Spitzack and Victor M. UgazPolymerase Chain Reaction on MicrochipsMaria C. Carles and Nikolaus J. SucherA Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle ProbesXiaojun Zhao, David T. Pierce, and Yanfu HuanMicrofluidic Ethanol Biobatteries on a MicrochipShelley D. Minteer and Christine M. MooreMicrofluidic Chambers for Cell Migration and Neuroscience ResearchAnne M. Taylor, Seog Woo Rhee, and Noo Li JeonMicrotextured Polydimethylsiloxane Substrates for Culturing Mesenchymal Stem CellsErik T. K. Peterson and Ian PapautskyContraction Study of a Single Cardiac Muscle Cell in a Microfluidic ChipXiujun (James) Li and Paul C. H. LiMicroscale Integrated Sperm SorterYaokuang Chung, Xiaoyue Zhu, Wei Gu, Gary D. Smith, and Shuichi TakayamaIndex