Topics In Fluorescence Spectroscopy Nonlinear And Two-Photon-Induced Fluorescence 1997 Edition

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Springer Topics In Fluorescence Spectroscopy Nonlinear And Two-Photon-Induced Fluorescence 1997 Edition by Joseph R. Lakowicz

Fluorescence spectroscopy continues its advance to more sophisticated methods and applications. As one looks over the previous decades its appears that the first practical instruments for time-resolved measurements appeared in the 1970's. The instrumentation and analysis methods for time-resolved fluorescence advanced rapidly throughout the 1980's. Since 1990 we have witnessed a rapid migration of the principles of time-resolved fluorescence to cell biology and clinical appli- tions. Most recently we have seen the introduction of multi-photon excitation pump-probe and stimulated emission methods for studies of biological mac- molecules and for cellular imaging. These advanced topics are the subject of the present volume. Two-photon excitation was first predicted by Maria Goppert-Mayer in 1931 but was not experimentally observed until 1961. Observation of two-photon excitation required the introduction of lasers which provided adequate photon density for multi-photon absorption. Since the early observations of two-photon excitation in the 1960s multi-photon spectroscopy has been limited to somewhat exotic applications of chemical physics where it is used to study the electronic symmetry of small molecules. Placing one's self back in 1980 it would be hard to imagine the use of multi-photon excitation in biophysics or cellular imaging. Table of contents : The Theory of Two-Photon Induced Fluorescence Anisotropy;P.R. Callis. Anisotropy Decays Induced by Two-Photon Excitation; C.K. Johnson C. Wan. Multiphoton Excitation of Biochemical Fluorophores; J.R. Lakowicz I. Gryczynski. Two-Photon Excitation and Anisotropy Decays in Membranes and Oriented Systems; W. van der Meer S.-Y.S. Chen. Two-Photon Induced Fluorescence of Proteins; b. Kierdaszuk et al. Fluorescence and Multiwave Mixing Induced by Photon Absorption of Excited Molecules; V. Bogdanov. Ultrafast Stimulated Emission Spectroscopy; G.J. Blanchard. Fluorescence Quenching by Stimulated Emission; J.R. Lakowicz I. Gryczynski. Increasing the Resolution of Far-Field Fluorescence Light Microscopy by Point Spread Functional Engineering; S.W. Hell. Time-Resolved Stimulated-Emission and Transient-Absorption Microscopy and Spectroscopy; P.T.C. So et al. Index.