Description
Nova Science Small Interfering RNA New Research 2009 Edition by Kaito Yamada
RNA interference is a gene silencing phenomenon triggered by dsRNA (double-stranded RNA) and has been widely used for studying gene functions. The short interfering RNA (siRNA) responsible for RNA interference varies markedly in its gene silencing efficacy. This book reviews the recently reported siRNA design guidelines and clarifies the problems concerning the guidelines. An effective method for selecting siRNA target sequences from many possible candidate sequence is also described by using Bayes' theorem. Furthermore, the development of a durable RNAi therapy for cancer and viral infections, which may require the use of multiple inhibitors is evaluated. This book also reviews the structure, application and therapeutic challenges of siRNA. Table of contents :- Preface; Silencing Persistent Virus Infections By RNA Interference; An Effective Method For Selecting siRNA Sequences By Using Bayes 'Theorem; Short Interfering RNA For Antiviral Treatment; Combinatorial RNAi Approaches: Killing 1 Bird With 2 Stones; RNAi, a Powerful Research Tool and Potential Molecular Therapy for Liver Diseases; Methylation and Loss of Heterozygosity in Cancer are linked by siRNA?; Biodegradable Poly (B-Amino Ester) Derivatives for Gene and siRNA Delivery; Evaluation of Published Rational siRNA Design Algorithms Using Firefly Luciferase Gene as a Reporter; Optimal siRNA Sequence Design for RNA Interference; Polymers for siRNA Delivery in Vivo; Index.