Description
Humana Press Rna Silencing Methods And Protocols by Gordon Carmichael
A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.show more Identification of Components of RNAi Pathways Using the Tandem Affinity Purification MethodMikiko C. Siomi and Haruhiko SiomiSeparation of Drosophila RNA Silencing Complexes by Native Gel ElectrophoresisJohn W. Pham and Erik J. SontheimerPreparation and Analysis of DroshaYoontae Lee and V. Narry KimRNA Interference in Caenorhabditis elegansNathaniel R. Dudley and Bob GoldsteinDown-Regulating Gene Expression by RNA Interference in Trypanosoma bruceiChristine E. Clayton, Antonio M. Estevez, Claudia Hartmann, Vincent P. Alibu, Mark Field, and David HornAnalysis of Double-Stranded RNA and Small RNAs Involved in RNA-Mediated Transcriptional Gene SilencingM. Florian Mette, Werner Aufsatz, Tatsuo Kanno, Lucia Daxinger, Philipp Rovina, Marjori Matzke, and Antonius J. M. MatzkeStrategies for the Design of Random siRNA Libraries and the Selection of Anti-GFP siRNAsMouldy Sioud and Sebastien WalchliSilencing Gene Expression with Dicer-Generated siRNA PoolsJason W. Myers and James E. Ferrell, Jr.Inhibition of Gene Expression In Vivo Using Multiplex siRNASung-Suk Chae and Timothy HlaGene Silencing by a DNA Vector-Based RNAi TechnologyGuangchao Sui and Yang ShiPlasmid-Based RNA Interference: Construction of Small-Hairpin RNA Expression VectorsScott Q. Harper and Beverly L. DavidsonsiRNA Delivery In VivoMouldy SioudPeptide-Based Strategy for siRNA Delivery into Mammalian CellsFederica Simeoni, May C. Morris, Frederic Heitz, and Gilles DivitaLentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic CellsMingjie Li and John J. RossiExon-Specific RNA Interference: A Tool to Determine the Functional Relevance of Proteins Encoded by Alternatively Spliced mRNAsAlicia M. Celotto, Joo-WonLee, and Brenton R. GraveleyImmunoprecipitation of MicroRNPs and Directional Cloning of MicroRNAsElisavet Maniataki, Maria Dels Angels De Planell Saguer, and Zissimos MourelatosDetection of MicroRNAs and Assays to Monitor MicroRNA Activities In Vivo and In VitroMarianthi Kiriakidou, Peter Nelson, Stella Lamprinaki, Anup Sharma, and Zissimos MourelatosHigh-Throughput Analysis of MicroRNA Gene Expression Using Sensitive ProbesMouldy Sioud and Oystein RosokModification of Human U1 snRNA for Inhibition of Gene Expression at the Level of Pre-mRNAPeng Liu, Mary L. Stover, Alexander Lichtler, and David W. RoweIndex