Description
Springer Quantification of Circulating Proteins Theory and applications based on analysis of plasma protein levels 1st Editon 2011 Softbound by Wim Th. Hermens, George M. Willems, Marja P. Visser
Less than 50 years ago it was discovered that steady-state protein concentrations in plasma are the net result of continuous elimination and synthesis of protein molecules. The first quanti tative studies on the turnover and distribution of plasma pro teins were made around 1950, after the introduction of radio labeled protein preparations. Around 1970, another development in quantitative interpre tation of circulating proteins was initiated in clinical enzy mology. Estimation of cumulative release into plasma of cellular enzymes can be helpful in a variety of diseases to assess the extent of tissue damage and to evaluate therapy. Enzymes can be considered as biological tracers, i.e. minute quantities of protein can be accurately determined by their spe cific catalytic activities. However, radioactive tracers permit direct estimates of turnover and distr ibution by measurement of excreted radioactivity, possibilities that are not available for enzymes. Consequently, only a few techniques used in tracer studies with radiolabeled proteins can be applied to circulating tissue enzymes and this may explain the lack of communication between the fields of plasma protein metabolism and quantitative clinical enzymology. In the present study a summary is given of the basic methods used in both fields, with emphasis on the equivalence of various models and formalisms used by different authors. It is shown that major limitations in the study of circulating tissue enzymes can be overcome if two different, but simultaneously released, en zymes can be measured. The resulting method will also be applied to plasma protein metabolism. 1: Introduction.- 1.1 Quantitative description of circulating proteins.- 1.2 Presentation of theory and applications.- A: Physiological basis, choice of models and quantitative methods.- 2: Turnover and distribution of circulating tissue enzymes.- 3: Turnover and distribution of plasma proteins.- 4: Methods and models used in quantitative studies of circulating proteins.- 5: Sources of error in quantification of circulating proteins.- B : Applications.- 6: Assessment of myocardial damage from plasma levels of cardiac enzymes.- 7: Changes in circulating mass of plasma proteins during the acute phase response.- 8: Dose-calculation of blood coagulation factors for infusion therapy.- 9: A review of data on the turnover and distribution of circulating proteins.- Appendix: Derivation of mathematical expressions.- References.