Description
Humana Protein Nmr Techniques 2E by A. Kristina Downing
When I was asked to edit the second edition of Protein NMR Techniques, my first thought was that the time was ripe for a new edition. The past several years have seen a surge in the development of novel methods that are truly revolutionizing our ability to characterize biological macromolecules in terms of speed, accuracy, and size limitations. I was particularly excited at the prospect of making these techniques accessible to all NMR labs and for the opportunity to ask the experts to divulge their hints and tips and to write, practically, about the methods. I commissioned 19 chapters with wide scope for Protein NMR Techniques, and the volume has been organized with numerous themes in mind. Chapters 1 and 2 deal with recombinant protein expression using two organisms, E. coli and P. pastoris, that can produce high yields of isotopically labeled protein at a reasonable cost. Staying with the idea of isotopic labeling, Chapter 3 describes methods for perdeuteration and site-specific protonation and is the first of several chapters in the book that is relevant to studies of higher molecular weight systems. A different, but equally powerful, method that uses molecular biology to "edit" the spectrum of a large molecule using segmental labeling is presented in Chapter 4. Having successfully produced a high molecular weight target for study, the next logical step is data acquisition. Hence, the final chapter on this theme, Chapter 5, describes TROSY methods for stru- ural studies. Screening and Optimizing Protein Production in E. coliLorraine Hewitt and James M. McDonnellIsotopic Labeling of Recombinant Proteins From the Methylotrophic Yeast Pichia pastorisAndrew R. Pickford and Joanne M. O'LearyPerdeuteration/Site-Specific Protonation Approaches for High-Molecular-Weight ProteinsStephen MatthewsSegmental Isotopic Labeling for Structural Biological Applications of NMRDavid Cowburn, Alexander Shekhtman, Rong Xu, Jennifer J. Ottesen, and Tom W. MuirTROSY-Based Correlation and NOE Spectroscopy for NMR Structural Studies of Large ProteinsGuang Zhu, Youlin Xia, Donghai Lin, and Xiaolian GaoMedia for Studies of Partially Aligned StatesKieran Fleming and Stephen MatthewsResidual Dipolar Couplings in Protein Structure DeterminationEva de Alba and Nico TjandraProjection Angle Restraints for Studying Structure and Dynamics of BiomoleculesChristian Griesinger, Wolfgang Peti, Jens Meiler, and Raphael BruschweilerCharacterizing Domain Interfaces by NMRLuke M. Rooney, Sachchidanand, and Joern M. WernerCharacterization of the Overall Rotational Diffusion of a Protein From 15N Relaxation Measurements and Hydrodynamic CalculationsJennifer Blake Hall, Olivier Walker, and David FushmanTROSY-Based NMR Experiments for the Study of Macromolecular Dynamics and Hydrogen BondingGuang Zhu, Youlin Xia, Donghai Lin, and Xiaolian GaoMeasurement of Intermediate Exchange PhenomenaJames G. Kempf and J. Patrick LoriaNMR Studies of Partially Folded Molten-Globule StatesChristina RedfieldStructure Determination of Protein Complexes by NMRDaniel Nietlispach, Helen R. Mott, Katherine M. Stott, Peter R. Nielsen, Abarna Thiru, and Ernest D. LaueNMR Studies of Protein-Nucleic Acid InteractionsGabriele Varani, Yu Chen, andThomas C. LeeperUsing NMRView to Visualize and Analyze the NMR Spectra of MacromoleculesBruce A. JohnsonAutomated NMR Structure Calculation With CYANAPeter GuntertNOE Assignment With ARIA 2.0: The Nuts and BoltsMichael Habeck, Wolfgang Rieping, Jens P. Linge, and Michael NilgesMembrane Protein Structure Determination Using Solid-State NMRAnthony Watts, Suzana K. Straus, Stephan L. Grage, Miya Kamihira, Yuen Han Lam, and Xin ZhaoIndex