Plant Transformation via Agrobacterium Tumefaciens Culture Conditions, Recalcitrance and Advances in Soybean 1st Edition at Meripustak

Plant Transformation via Agrobacterium Tumefaciens Culture Conditions, Recalcitrance and Advances in Soybean 1st Edition

Books from same Author: Phetole Mangena

Books from same Publisher: CRC Press

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  • General Information  
    Author(s)Phetole Mangena
    PublisherCRC Press
    Edition1st Edition 2022
    ISBN9781032250380
    Pages244
    BindingSoftbound
    LanguageEnglish
    Publish YearSeptember 2022

    Description

    CRC Press Plant Transformation via Agrobacterium Tumefaciens Culture Conditions, Recalcitrance and Advances in Soybean 1st Edition by Phetole Mangena

    Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes.
    This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment.
    Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.

    Table of Contents:-

    Overview of Genetic Transformation in Plants
    Plant Transformation History
    Agrobacterium Tumefaciens
    Getting Started with In Vitro Soybean Transformation
    In Vitro Cultures Commonly used for Plant Transformation
    Current and Longstanding Challenges facing Soybean Transformation
    Alternative Techniques for Genetic Manipulations via A. Tumefaciens
    Molecular Aspects of Indirect Gene Transfer via A. Tumefaciens
    Genetic Transformation in Other Leguminous Crops
    Transgenic Plants for Biotic Stress Resistance
    Transgenic Plants for Abiotic Stress Resistance
    Potential Health Safety Concerns and Environmental Risks
    The Regulatory Management of Transgenic Plants
    Current and Future Prospects in Genetic Engineering
    Photographic Index of Soybean Transformation Culture

    About the Author:-
    Phetole Mangena obtained his PhD degree in Botany in the field of plant physiology and biotechnology. He is a senior lecturer and researcher in the Department of Biodiversity, School of Molecular and Life Sciences, Faculty of Science and Agriculture at the University of Limpopo, Republic of South Africa. Dr Mangena’s work involve micropropagation via tissue culture, crop improvement and breeding through mutagenic chemicals as well as Agrobacterium-mediated genetic transformation. He has authored and co-authored numerous scientific papers and book chapters related to genetic transformation and morpho-physiological responses of plants to stress. Dr. Mangena is the sole editor of the Book: Advances in Legume Research- Physiological Responses and Genetic Improvement for Stress Resistance, published in 2020 (Volume 1) by Bentham Science Publishers Pte. Ltd, Singapore. He currently serves as the editor for the Scopus indexed Research Journal of BioTechnology and a fellow-member of the World Researchers Association. He is also a board member of the New Plant Biotechnology Persa Journal, member of the South African Association of Botanists and the International Society for Horticultural Science. Dr. Mangena has received numerous honours and awards, including 1st prize in the Innovation Hub GAP Bioscience Competition and the Research Excellence Award by the National Research Foundation of South Africa.