New And Emerging Proteomic Techniques

Description

Springer New And Emerging Proteomic Techniques by Dobrin Nedelkov , Randall W. Nelson

Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls._x000D_ _x000D_On-Chip Protein Synthesis for Making Microarrays_x000D_ _x000D_ Niroshan Ramachandran, Eugenie Hainsworth, Gokhan Demirkan, and Joshua LaBaer_x000D_ _x000D_ _x000D_ _x000D_ RCA-Enhanced Protein Detection Arrays_x000D_ _x000D_ Brian B. Haab and Paul M. Lizardi_x000D_ _x000D_ _x000D_ _x000D_ Antibody Microarrays Using Resonance Light-Scattering Particles for Detection_x000D_ _x000D_ Bernhard H. Geierstanger, Petri Saviranta, and Achim Brinker_x000D_ _x000D_ _x000D_ _x000D_ Chemical Proteomics Profiling of Proteasome Activity_x000D_ _x000D_ Martijn Verdoes, Celia R. Berkers, Bogdan I. Florea, Paul F. van Swieten, Herman S. Overkleeft, and Huib Ovaa_x000D_ _x000D_ _x000D_ _x000D_ Two-Dimensional Difference Gel Electrophoresis_x000D_ _x000D_ Terence L. Wu_x000D_ _x000D_ _x000D_ _x000D_ Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors_x000D_ _x000D_ Ewa Folta-Stogniew_x000D_ _x000D_ _x000D_ _x000D_ Surface Plasmon Resonance Imaging Measurements of Protein Interactions With Biopolymer Microarrays_x000D_ _x000D_ Terry T. Goodrich, Alastair W. Wark, Robert M. Corn, and Hye Jin Lee_x000D_ _x000D_ _x000D_ _x000D_ Surface Plasmon Resonance Mass Spectrometry for Protein Analysis_x000D_ _x000D_ Dobrin Nedelkov and Randall W. Nelson_x000D_ _x000D_ _x000D_ _x000D_ High-Throughput Affinity Mass Spectrometry_x000D_ _x000D_ Urban A. Kiernan, Dobrin Nedelkov, Eric E. Niederkofler, Kemmons A. Tubbs, and Randall W. Nelson_x000D_ _x000D_ _x000D_ _x000D_ Isotope-Coded Affinity Tags for Protein Quantification_x000D_ _x000D_ Christopher M. Colangelo and Kenneth R. Williams_x000D_ _x000D_ _x000D_ _x000D_ Proteomic Analysis by Multidimensional Protein Identification Technology_x000D_ _x000D_ Laurence Florens and Michael P. Washburn_x000D_ _x000D_ _x000D_ _x000D_ Isolation of Glycoproteins and Identification of Their N-Linked Glycosylation Sites_x000D_ _x000D_ Hui Zhang and Ruedi Aebersold_x000D_ _x000D_ _x000D_ _x000D_ N-Glycosylation Analysis Using the StrOligo Algorithm_x000D_ _x000D_ Martin Ethier, Daniel Figeys, and Helene Perreault_x000D_ _x000D_ _x000D_ _x000D_ MALDI-MS Data Analysis for Disease Biomarker Discovery_x000D_ _x000D_ Weichuan Yu, Baolin Wu, Junfeng Liu, Xiaoye Li, Kathy Stone, Kenneth R. Williams, and Hongyu Zhao_x000D_ _x000D_ _x000D_ _x000D_ Using the Global Proteome Machine for Protein Identification_x000D_ _x000D_ Ronald C.Beavis_x000D_ _x000D_ _x000D_ _x000D_ Index_x000D_