Description
Humana Press Inc. Molecular Motors Methods And Protocols by Ann O. Sperry
Molecular motor proteins produce force for movement in an incredibly wide variety of cellular processes. This volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family. In addition, it describes techniques directed at motors that fall outside of the three characterized families: dynamin and F1ATPase._x000D_ _x000D_
Contents_x000D_
_x000D_
Preface_x000D_
Contributors _x000D_
_x000D_
CH. 1 Equilibrium Binding of Proteins to F-Actin, Joseph M. Chalovich_x000D_
CH. 2 Analysis of Calcium/Calmodulin Regulation of a Plant Kinesin_x000D_
Using Cosedimentation and ATPase Assays, Anireddy S.N. Reddy _x000D_
CH. 3 In Vitro and In Vivo Analysis of Microtubule Destabilizing_x000D_
Kinesins, Jason Stumpff, Jeremy Cooper, Sarah Domnitz, Ayana Moore,_x000D_
Kathleen Rankin, Mike Wagenbach and Linda Wordeman _x000D_
CH. 4 Approaches to Kinesin-1 Phosphorylation, Gerardo Morfini, Gustavo_x000D_
Pigino, and Scott T. Brady _x000D_
CH. 5 Protein Modification to Probe Intra-dynein Interactions and In_x000D_
Vivo Redox State, Ken-ichi Wakabayashi, Miho Sakato and Stephen M._x000D_
King _x000D_
CH. 6 Methods to Study the Interactions of the Dynein Light Chains and_x000D_
Intermediate Chains, Kevin W.-H. Lo and K. Kevin Pfister _x000D_
CH. 7 Identification of Motor Protein Cargo by Yeast 2-Hybrid And_x000D_
Affinity Approaches, Yuguo Zhang, Rong Wang, Holly Jefferson, and Ann_x000D_
O. Sperry _x000D_
CH. 8 In Situ Binding Assay to Measure Myosin-1c Interactions with_x000D_
Hair-Cell Proteins, Kelli R. Phillips and Janet L. Cyr _x000D_
CH. 9 Ultrastructural Analysis of Kinesin-related Motor Proteins During_x000D_
Spermatogenesis, Wan-Xi Yang _x000D_
CH. 10 In Vitro Motility System to Study the Role of Motor Proteins in_x000D_
Receptor-Ligand Sorting, John W. Murray and Allan W. Wolkoff _x000D_
CH. 11 Enrichment and Disassembly of Ectoplasmic Specializations in the_x000D_
Rat Testis, Julian A. Guttman, Kuljeet S.Vaid and A. Wayne Vogl_x000D_
CH. 12 Single-Molecule Observation of Rotation of F1-ATPase through_x000D_
Micro Beads, Takayuki Nishizaka, Kana Mizutani and Tomoko Masaike _x000D_
CH. 13 The use of FRET in the Analysis of Motor Protein Structure,_x000D_
Andre Kasprzak _x000D_
CH. 14 Structure Determination of the Motor Domain of Yeast Kinesin_x000D_
Kar3 by X-ray Crystallography, Hee-Won Park _x000D_
CH. 15 High Resolution Structural Analysis of the Kinesin-Microtubule_x000D_
Complex by Electron Cryo-Microscopy, Keiko Hirose and LindaA. Amos _x000D_
CH. 16 Chemical-Genetic Inhibition of Sensitized Mutant Unconventional_x000D_
Myosins, Ryan L. Karcher, D. William Provance, Jr., Peter G. Gillespie,_x000D_
and John A. Mercer_x000D_