Description
Humana Press Inc. In Vitro Transcription And Translation Protocols by Guido Grandi
A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included._x000D_ _x000D_
Section One: TECHNOLOGIES _x000D_
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Chapter One - Energy Systems for ATP Regeneration in Cell-free Protein Synthesis Reactions_x000D_
Kara A. Calhoun, James R. Swartz_x000D_
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Chapter 2 - Continuous-exchange Protein-synthesizing Systems_x000D_
Vladimir A. Shirokov, Aigar Kommer, Vyacheslav A. Kolb, Alexander S. Spirin _x000D_
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Chapter 3 - Cell-free production of integral membrane proteins on a preparative scale'_x000D_
Christian Klammt, Daniel Schwarz, Vilker Doetsch, Frank Bernhard_x000D_
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Chapter 4- SIMPLEX: Single-molecule PCR-linked in vitro expression: a novel method for high-throughput construction and screening of protein libraries_x000D_
Suang Rungpragayphan, Tsuneo Yamane, Hideo Nakano_x000D_
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Chapter 5 - Methods for High-throughput Materialization of Genetic Information Based on Wheat Germ Cell-free Expression System _x000D_
Tatsuya Sawasaki, Ryo Morishita, Mudeppa D. Gouda, Yaeta Endo_x000D_
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Chapter 6 - Exogenous Protein Expression in Xenopus Oocytes: Basic Procedures_x000D_
Elena Bossi, Maria Serena Fabbrini, Aldo Ceriotti_x000D_
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Section Two: APPLICATIONS_x000D_
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Chapter 7 -In vitro translation to study HIV protease activity_x000D_
Zene Matsuda, Mutsunori Iga, Kosuke Miyauchi, Jun Komano, Kazuhiro Morishita, Akihiko Okayama, Hirohito Tsubouchi_x000D_
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Chapter 8 - The protein truncation test in mutation detection and molecular diagnosis_x000D_
Oliver Hauss, Oliver Muller_x000D_
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Chapter 9 - Creation of Novel Enantioselective lipases by SIMPLEX_x000D_
Yuichi Koga, Tsuneo Yamane, Hideo Nakano_x000D_
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Chapter 10 - In vitro transcription and translation coupled to 2D-electrophoresis for bacterial proteome analysis_x000D_
Nathalie Norais, Ignazio Garaguso, Germano Ferrari, Guido Grandi_x000D_
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Chapter 11 - In vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation and Immunoprecipitation Analysis_x000D_
Orit Gat, Haim Grosfeld,Avigdor Shafferman _x000D_
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Chapter 12 - Functional expression of type 1 rat GABA transporter in microinjected Xenopus laevis oocytes_x000D_
Stefano Giovannardi, Simona Magagnin, Andrea Soragna, Laura Faravelli_x000D_
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Chapter 13 - Production of Protein for NMR Study Using the Wheat Germ Cell-Free System_x000D_
Toshiyuki Kohno, Yaeta Endo_x000D_