Immunological Tolerance Methods And Protocols

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Humana Press Inc. Immunological Tolerance Methods And Protocols by Paul J. Fairchild

Immunological Tolerance: Methods and Protocols is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo. This guide is aimed at AIDS researchers, immunologists, transplant surgeons/specialists, and biotechnologists._x000D_ _x000D_ Immunological Tolerance: Methods and Protocols_x000D_ Table of Contents_x000D_ _x000D_ Introduction_x000D_ 1. Frontiers of Immunological Tolerance_x000D_ Giorgio Raimondi, Heth Turnquist & Angus Thomson_x000D_ University of Pittsburgh Medical Center, Thomas E Starzl Transplantation Institute, Pittsburgh, USA_x000D_ _x000D_ Cell Types Contributing to Immunological Tolerance_x000D_ 2. Case Study: Balancing Tolerance & Immunity: The Role of Dendritic Cell & T Cell Subsets_x000D_ Elena Chklovskaia & Barbara Fazekas de St Groth_x000D_ Centenary Institute of Cancer Medicine & Cell Biology, University of Sydney, Sydney, Australia_x000D_ _x000D_ 3. Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow_x000D_ Ludovica Bruno_x000D_ The Institute of Cancer Research, London, UK_x000D_ _x000D_ 4. Genetic Modification of Dendritic Cells through the Directed Differentiation of Embryonic Stem Cells_x000D_ Paul J. Fairchild, Kathleen F. Nolan, &Herman Waldmann_x000D_ Sir William Dunn School of Pathology, University of Oxford, Oxford, UK_x000D_ _x000D_ 5. Generation of Immunocompetent T Cells from Embryonic Stem Cells_x000D_ Renee F. de Pooter & Juan-Carlos Zuniga-Pflucker_x000D_ Dept of Immunology, University of Toronto, Sunnybrook & Women's Research Institute, Toronto, Canada_x000D_ _x000D_ 6. Isolation, Expansion and Characterization of Human Natural and Adaptive Regulatory T Cells_x000D_ Silvia Gregori, Rosa Bacchetta,Laura Passerini, Megan K. Levings & Maria Grazia Roncarolo _x000D_ San Raffaele Telethon Institute for Gene Therapy, Milan, Italy_x000D_ _x000D_ 7. Derivation, Culture and Characterization of Thymic Epithelial Cell Lines_x000D_ Michiyuki Kasai & Toshiaki Mizuochi_x000D_ Dept of Research on Blood & Biological Product, National Institute of Infectious Diseases, Tokyo, Japan_x000D_ _x000D_ The Study of Immunological Tolerance in Vitro_x000D_ 8. Case Study: Thymus Organogenesis and Development of the Thymic Stroma_x000D_ Craig Scott Nowell, Alison Mary Farley & Catherine Clare Blackburn_x000D_ Institute for Stem Cell Research, University of Edinburgh, Edinburgh, UK_x000D_ _x000D_ 9. Case Study: Generation of a Tissue Engineered Thymic Organoid_x000D_ Fabrizio Vianello & Mark C. Poznansky_x000D_ Infectious Diseases Division & Partners AIDS Research Center, Harvard Medical School, Charlestown, USA_x000D_ _x000D_ 10. Studying T Cell Repertoire Selection Using Fetal Thymus Organ Cultures_x000D_ Philip G. Ashton-Rickardt_x000D_ Department of Pathology & Ben May Institute for Cancer Research, University of Chicago, Chicago, USA_x000D_ _x000D_ 11. Investigating Central Tolerance with Reaggregate Thymus Organ Cultures_x000D_ Graham Anderson & Eric J. Jenkinson_x000D_ MRC Centre for Immune Regulation, Department of Anatomy, University of Birmingham, Birmingham, UK_x000D_ _x000D_ 12. Estimating Thymic Function through Quantification of T Cell Receptor Excision Circles_x000D_ Marie-Lise Dion, Rafick-Pierre Sekaly & Remi Cheynier_x000D_ Laboratoire d'Immunologie, Centre de Recherches du CHUM, Montreal, Canada_x000D_ _x000D_ 13. Gene Expression Profiling of Dendritic Cells by Microarray_x000D_ Maria Foti, Paola Ricciardi-Castagnoli & Francesca Granucci_x000D_ Dept of Biotechnology and Bioscience, University of Milano-Bicocca, Milan, Italy_x000D_ _x000D_ 14. SAGE Analysis of Cell Types Involved in Tolerance Induction_x000D_ Kathleen F. Nolan, Stephen P. Cobbold & Herman Waldmann_x000D_ Sir William Dunn School of Pathology, University of Oxford, Oxford, UK_x000D_ _x000D_ 15. Analyzing the Physicodynamics of Immune Cells in a 3-D Collagen Matrix_x000D_ Peter Reichardt, Frank Gunzer & Matthias Gunzer_x000D_ German Research Centre for Biotechnology, Braunschweig, Germany_x000D_ _x000D_ The Study of Immunological Tolerance in Vivo_x000D_ 16. Case Study: Aetiology of Autoimmune Disease: How T Cells Escape Self-Tolerance_x000D_ Eli Sercarz & Claudia Raja-Gabaglia_x000D_ Torey Pines Institute for Molecular Studies, San Diego, USA_x000D_ _x000D_ 17. Case Study: Animal Models of Spontaneous Autoimmune Disease: Type 1 Diabetes_x000D_