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Handbook Of Elispot: Methods And Protocols at Meripustak

Handbook Of Elispot: Methods And Protocols

Books from same Author: Alexander E. Kalyuzhny

Books from same Publisher: Humana Press Inc.

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    • General Information  
    Author(s)Alexander E. Kalyuzhny
    PublisherHumana Press Inc.
    Edition..
    ISBN9781588294692
    Pages336
    BindingHardback
    LanguageEnglish
    Publish YearApril 2005

    Description

    Humana Press Inc. Handbook Of Elispot: Methods And Protocols by Alexander E. Kalyuzhny

    In this first book dedicated entirely to the ELISPOT a critical enzyme-linked immunospot assay used widely in biomedical research recognized experts with first-hand experience detail how to design perform and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics including the use of membrane-backed plates the standardization and validation procedures the removal of cells from ELISPOT plates cell separation techniques and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models human cells measles multiple sclerosis immune responses multicytokine detection systems and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays use of the ELISPOT assay on feline lymphocytes standardization of the ELISPOT procedure and combining the ELISPOT assay with immunohistochemistry.