Description
Humana Gene Delivery To Mammalian Cells Volume 2 Viral Gene Transfer Techniques by William C. Heiser
The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology-assessment of gene function and gene therapy-require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery. Part I. Delivery Using AdenovirusesAdenovirus-Mediated Gene Delivery: An OverviewJoanne T. DouglasDNA Delivery to Cells in Culture: Generation of Adenoviral Libraries for High-Throughput Functional ScreeningMiroslava Ogorelkova, Seyyed Mehdy Elahi, David Gagnon, and Bernard MassieAdenovirus-Mediated Gene Delivery to Skeletal MuscleJoanne T. DouglasDelivery of Adenoviral DNA to Mouse LiverSheila Connelly and Christine MechDelivery of DNA to Lung Airway EpitheliumDaniel J. WeissDelivery of DNA to Pulmonary Endothelium Using Adenoviral VectorsPaul N. ReynoldsGene Transfer to Brain and Spinal Cord Using Recombinant Adenoviral VectorsJoseph M. Alisky and Beverly L. Davidson.Adenovirus-Mediated Gene Transfer to Tumor CellsManel Cascallo and Ramon AlemanyAdenovirus-Mediated Gene Delivery to Dendritic CellsLaura Timares, Joanne T. Douglas, Bryan W. Tillman, Victor Krasnykh, and David T. CurielPart II. Delivery Using Adeno-Associated VirusesOverview of Adeno-Associated Viral VectorsThomas M. DalyAAV Vector Delivery to Cells in CultureAndrew Smith, Roy Collaco, and James P. TrempeAAV-Mediated Gene Transfer to Skeletal MuscleRoland W. HerzogAAV-Mediated Gene Transfer to the LiverThomas M. Daly. AAV-Mediated Gene Transfer to Mouse Lungs, Christine L. Halbert and A. Dusty MillerGene Delivery to the Mammalian Heart Using AAV VectorsDanny Chu, Patricia A. Thistlethwaite, Christopher C. Sullivan, Mirta S. Grifman, and Matthew D. WeitzmanGene Delivery to the Mouse Brain with Adeno-Associated VirusMarco A. Passini, Deborah J. Watson, and John H. WolfeDelivery of DNA to Tumor Cells In Vivo Using Adeno-Associated VirusSelvarangan Ponnazhagan and Frank HooverGene Delivery toHuman and Murine Primitive Hematopoietic Stem and Progenitor Cells by AAV2 VectorsArun SrivastavaPart III. Delivery Using Herpes Simplex VirusesDelivery Using Herpes Simplex Virus: An OverviewWilliam F. Goins, Darren Wolfe, David M. Krisky, Qing Bai, Ed A. Burton, David J. Fink, and Joseph C. GloriosoGene Transfer to Skeletal Muscle Using Herpes Simplex Virus-Based VectorsBaohong Cao and Johnny HuardDelivery of Herpes Simplex Virus-Based Vectors to the Nervous SystemJames R. Goss, Atsushi Natsume, Darren Wolfe, Marina Mata, Joseph C. Glorioso, and David J. FinkGene Transfer to Glial Tumors Using Herpes Simplex VirusAjay Niranjan, Darren Wolfe, Wendy Fellows, William F. Goins, Joseph C. Glorioso, Douglas Kondziolka, and L. Dade LunsfordDelivery of Herpes Simplex Virus-Based Vectors to Stem CellsDarren Wolfe, James B. Wechuck, David M. Krisky, Julie P. Goff, William F. Goins, Ali Ozuer, Michael E. Epperly, Joel S. Greenberger, David J. Fink, and Joseph C. GloriosoPart IV. Delivery Using BaculovirusesBaculovirus-Mediated Gene Delivery into Mammalian CellsRaymond V. Merrihew, Thomas A. Kost, and J. Patrick CondreayPart V. Delivery Using LentivirusesGene Delivery by Lentivirus Vectors: An OverviewTal KafriLentiviral Vectors for the Delivery of DNA into Mammalian CellsRoland Wolkowicz, Garry P. Nolan, and Michael A. CurranStable Gene Delivery to CNS Cells Using Lentiviral VectorsDeborah J. Watson, Brian A. Karolewski, and John H. WolfeGene Delivery to Hematopoietic Stem Cells Using Lentiviral VectorsHiroyuki MiyoshiDelivery of Genes to the Eye Using Lentiviral VectorsMasayo TakahashiLentiviral Transduction of Human Dendritic CellsRoland Schroers and Si-Yi ChenPart VI. Delivery Using Retroviruses