Description
Humana Press Inc. Biological Aging Methods And Protocols by Trygve O. Tollefsbol
This book investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions._x000D_ _x000D_
Contents:_x000D_
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Preface_x000D_
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Contributors_x000D_
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Techniques for Analysis of Biological Aging _x000D_
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Trygve O. Tollefsbol_x000D_
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Aging Cell Culture: Methods and Observations_x000D_
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Sharla M. O. Phipps, Joel B. Berletch, Lucy G. Andrews,_x000D_
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and Trygve O. Tollefsbol_x000D_
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Methods to Detect Biomarkers of Cellular Senescence: The Senescence Associated b-galactosidase _x000D_
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Koji Itahana, Judith Campisi, and Goberdhan P. Dimri_x000D_
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Methods of Cell Sorting of Young and Senescent Cells _x000D_
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Joao F. Passos and Thomas von Zglinicki_x000D_
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Telomere Length Analysis _x000D_
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Andres Canela, Peter Klatt, and Maria A. Blasco_x000D_
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A Method to Detect DNA Methyltransferase I Gene Transcription In Vitro in Aging Systems_x000D_
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Joel B. Berletch, Lucy G. Andrews, and Trygve O._x000D_
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Tollefsbol_x000D_
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A Method to Study the Expression of DNA Methyltransferases in Aging Systems In Vitro_x000D_
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Joel B. Berletch, Sharla M. O. Phipps, Sabrina L. Walthall, Lucy G. Andrews, and Trygve O. Tollefsbol_x000D_
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The Chronological Life Span of Saccharomyces Cerevisiae_x000D_
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Paola Fabrizio and Valter D. Longo_x000D_
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Caloric Restriction and Life-Span Determination of Yeast Cells _x000D_
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Oliver Medvedik and David A. Sinclair_x000D_
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Methods of Nutrigenomics and Longevity Studies in Drosophila: Effects of Diets High in Sucrose, Palmitic Acid, Soy or Beef _x000D_
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Jiatao Ye, Xiangqin Cui, Ann Loraine, Kerry Bynum, Nam Chul Kim, Gregory White, Maria De Luca, Mark D. Garfinkel, Xiangyi Lu, and Douglas M. Ruden _x000D_
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Caloric Restriction Mimetics: A Novel Approach for Biogerontology _x000D_
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Mark A. Lane, George S. Roth, and Donald K. Ingram_x000D_
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Extension of Cell Life-Span Using Exogenous Telomerase _x000D_
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Mo K. Kang and No-Hee Park_x000D_
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Inducing Cellular Senescence Using Defined Genetic Elements _x000D_
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Hiroshi Nakagawa and Oliver G. Opitz_x000D_
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Methods of Induction of Cellular Senescence Using Oxidative Stresslas Hales_x000D_
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Nuclear Transfer Methods to Study Aging _x000D_
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Lin Liu and David L. Keefe_x000D_
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Use of Ribozymes in Cellular Aging Research _x000D_
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Custer C. Deocaris, Sunil C. Kaul, Kazunari Taira, and Renu Wadhwa_x000D_
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Methods of Evaluation of Pharmacological Drugs Effects on Aging and Life Span in Mice _x000D_
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Vladimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski_x000D_
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Identifying Genes that Extend Life-Span Using a High Throughput Screening System _x000D_
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Cuiying Chen and Roland Contreras _x000D_
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Application of DNA Microarray Technology to Gerontological Studies _x000D_
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Sergey V. Anisimov_x000D_
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Detection and Analysis of Somatic Mutations at a LacZ Reporter Locus in Higher Organisms: Application to Mus Musculus and Drosophila Melanogaster _x000D_
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Ana Maria Garcia, Rita A. Busuttil, Armando Rodriguez, Carlos Cabrera, Martha Lundell, Martijn E. T. Dolle, and Jan Vijg _x000D_
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Subtractive Hybridization Techniques to Study Cellular Senescence _x000D_
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Michal B. Sternberg and Shimon Gepstein_x000D_
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Mapping Genetic Polymorphisms Affecting Natural Variation in Drosophila Longevity _x000D_
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Maria De Luca and Jeff Leips_x000D_
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Quantitative Trait Locus (QTL) Mapping in Aging Systems _x000D_
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Hui-Chen Hsu, Lu Lu, Nengjun Yi, Gary Van Zant, Robert W. Williams, and John D. Mountz_x000D_
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2D Gel Proteomics: An Approach to Study Age-related Differences in Protein-abundance or -Isoform Complexity in Biological Samples_x000D_
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Helen Kim, Shannon Eliuk, Jessy Deshane, Sreelatha Meleth, Todd Sanderson, Anita Pinner, Gloria Robinson, Landon Wilson, Marion Kirk, and Stephen Barnes_x000D_
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Metabolomics in the Study of Aging and Caloric Restriction _x000D_
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Bruce S. Kristal, Yevgeniya I. Shurubor, Rima Kaddurah- Daouk, and Wayne R. Matson_x000D_
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Index_x000D_