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Basic Cell Culture Protocols, 3Ed at Meripustak

Basic Cell Culture Protocols, 3Ed

Books from same Author: Cheryl D. Helgason

Books from same Publisher: Humana Press Inc.

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    • General Information  
    Author(s)Cheryl D. Helgason
    PublisherHumana Press Inc.
    Edition3rd Revised edition
    ISBN9781588295453
    Pages384
    BindingMixed media product
    LanguageEnglish
    Publish YearOctober 2004

    Description

    Humana Press Inc. Basic Cell Culture Protocols, 3Ed by Cheryl D. Helgason

    In this fully revised edition of an established classic expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types such as kidney proximal tubule cells hepatocytes keratinocytes and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types for fetal thymic organ culture and for the isolation and culture of specialized cell types such as mammary progenitor cells skeletal muscle myofibers mesenchymal cells neural stem cells hematopoietic cells stromal cell lines and endothelial cells. Additional chapters describe new techniques (leukocyte rolling isolation of side-population cells and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma DNA fingerprinting and cytogenetic analysis).