Adenovirus Methods And Protocols Volume 2 Ad Proteins And Rna Lifecycle And Host Interactions And Phyologenetics

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Humana Press Inc. Adenovirus Methods And Protocols Volume 2 Ad Proteins And Rna Lifecycle And Host Interactions And Phyologenetics by William S. M. Wold

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. The protocols presented are written by trendsetting researchers._x000D_ _x000D_ Ann E. Tollefson, Mohan Kuppuswamy, and William S. M. Wold_x000D_ TABLE OF CONTENTS_x000D_ Vol. II Topics: Ad proteins and RNA, lifecycle and host interactions, and phylogenetics_x000D_ _x000D_ 1. Analysis of the Efficiency of Adenovirus Transcription_x000D_ Cristina Iftode and S. J. Flint_x000D_ 2. The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domains_x000D_ Paul M. Loewenstein, Chao-Zhong Song and Maurice Green _x000D_ 3. Preparation of Soluble Extracts from Adenovirus Infected Cells for Studies of RNA Splicing_x000D_ Oliver Muhlemann and Goeran Akusjarvi_x000D_ 4. In Vitro Methods to Study RNA Interference During an Adenovirus Infection_x000D_ Gunnar Andersson, Ning Xu and Goeran Akusjarvi_x000D_ 5. Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situ_x000D_ Eileen Bridge_x000D_ 6. Study of Nucleolar Localization of Adenovirus Core Proteins_x000D_ David A. Matthews_x000D_ 7. Analysis of Adenovirus Infections in Synchronized Cells_x000D_ David A. Ornelles, Robin N. Shepard and Felicia D. Goodrum_x000D_ 8. Co-Immunoprecipitation of Protein Complexes_x000D_ Peter Yaciuk_x000D_ 9. Chromatin Immunoprecipitation (ChIP) to Study the Binding of Proteins to the Adenovirus Genome in Vivo_x000D_ Jihong Yang and Patrick Hearing_x000D_ 10. Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assays_x000D_ Pilar Perez-Romero and Michael J. Imperiale_x000D_ 11. Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry_x000D_ Anuj Gaggar, Dmitry Shayakhmetov, and Andre Lieber_x000D_ 12. The Use of Cell Microinjection for the in vivo Analysis of Viral Transcriptional Regulatory Protein Domains_x000D_ Maurice Green, Andrew Thorburn, Robert Kern and Paul M. Loewenstein_x000D_ 13. Determination of the Transforming Activities of Adenovirus Oncogenes_x000D_ Michael Nevels and Thomas Dobner_x000D_ 14. Human Adenovirus Type 12: Crossing Species Barriers toImmortalize the Viral Genome_x000D_ Walter Doerfler_x000D_ 15. Measurement of NK Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells _x000D_ John M. Routes_x000D_ 16. A Flow Cytometric Assay for Analysis of NK Cell-Mediated Cytolysis of Adenovirus-Transformed Cells_x000D_ Graham Bottley, Graham P. Cook, and G. Eric Blair_x000D_ 17. Large Scale Purification and Crystallization of Adenovirus Hexon _x000D_ John J. Rux and Roger M. Burnett_x000D_ 18. Synthesis and Assay of Recombinant Adenovirus Protease _x000D_ Joseph M. Weber_x000D_ 19. Assay for the Adenovirus Proteinase- Purification of the Enzyme and Synthesis of a Fluorogenic Substrate_x000D_ Walter F. Mangel and William J. McGrath_x000D_ 20. Cofactors of the Adenovirus Proteinase: Measuring Equilibrium Dissociation Constants and Stoichiometries of Binding _x000D_ Walter F. Mangel and William J. McGrath_x000D_ 21. Characterization of the Adenovirus Fiber Protein_x000D_ Jeffrey A. Engler and Jeong Shin Hong_x000D_ 22. Phylogenetic Analysis of Adenovirus Sequences_x000D_ Balazs Harrach and Maria Benkoe_x000D_ 23. Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology Studies_x000D_ Adriana E. Kajon and Dean D. Erdman_x000D_